Hi everyone,
Continuing on from my question of a couple of weeks ago. I have a milk by product "filter cake" that is highly insoluble. I need to get it into solution so that I can separate the peptides contained within it by HPLC and then sequence them.
I have managed to get the filter cake into solution by dissolving in 70% formic acid, which I then diluted to 35%. My question is this- will 35% formic acid affect (or even destroy) a C18 column or have any affect when mixed with acetonitrile?
Thanks alot
Amanda
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Amanda Hall-Griffin
Professional Officer
Newcastle Protein
University of Newcastle
Ph (02) 4921-7299
Fax (02) 4921-6903
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