Re: AAA: dimethyl Arg

From: Ming F. Tam (mbr405@ccvax.sinica.edu.tw)
Date: Mon Mar 19 2001 - 20:19:37 EST

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John:

We have done that. We have used the Pierce AA511 column and sodium citrate
buffer for elution. The dimethyl arginines eluted off the column after
50-60 minutes but the asymmetric and symmetric dimethyl arginines are too
close to be separated. We detect the peaks by ninhydrin. Alternatively,
we use the PicoTag system and a very shallow gradient to do the
separation. The mono- and the two dimethyl-arginines can be well
separated. You can write me for further details.

Ming

Ming F. Tam, Ph.D.
Research Scientist
Institute of Molecular Biology
Academia Sinica
Phone: 886-02-2788-3425
EMail: mbr405@ccvax.sinica.edu.tw

At 09:39 AM 2001/3/19 -0500, John Hempel wrote:

>I would appreciate hearing from anyone with experience analyzing dimethyl-Arg.
>
>Thanks,
>John
>John Hempel, PhD Ph (412) 624 0161
>University of Pittsburgh FAX (412) 624 4759
>Department of Biological Sciences
>Clapp Hall 301
>Pittsburgh PA 15260 USA
>
>email: hempel@psc.edu
>http://www.pitt.edu/~biology/faculty/hempel.html

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