At pH 5.2 one would not used TFA.I have not used any stuff like that in
years,but I suspect that the buffer is sodium acetate.Its concentration is
important to separate H and R.And there may be some tetrahydrofuran as
well.
Fulvio Perini,Ph.D.
986805 Nebraska Med.Cntr.
Omaha,NE 68198-6805
ph:402-559-6184/FAX 402-559-4651
fperini@unmc.edu
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