Re: Agilent Sequencer N-terminal Mobile Phase A

From: Jelle Lahnstein (jelle.lahnstein@adelaide.edu.au)
Date: Wed Mar 21 2001 - 00:06:08 EST


Here is a an alternative eluent (A), it is not identical to Agilent's (A) but
gives a very similar chromatogram. I have found it does not give good results
on columns that have already been used with Agilent's eluents.

1g/L heptanesulphonic acid sodium salt mono-hydrate
160 mL/L acetonitrile
1.5 mL/L acetic acid
276 uL/L triethylamine
water to 1 L

adjust to pH 5.5 with 10M sodium hydroxide (~ 2 mL)

--
Jelle Lahnstein

Department of Plant Science Waite Campus, The University of Adelaide Urrbrae, South Australia 5064 Tel: +61 8 83037260 Fax: +61 8 83037130 http://planta.waite.adelaide.edu.au/research.htm



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