Amos -
Wouldn't the anomer separation be a problem at low pH but not at high? See
J. Chromatogr. A, 676 (1994) 191-202. A mobile phase containing 0.1%
triethylamine would probably speed up the rate of mutarotation sufficiently
to afford a single symmetrical peak for the anomers.
Best regards,
Andy Alpert
PolyLC Inc.
(410) 992-5400
************************************
<< Subj: Re: CHO - who does glucosamine analysis?
Date: 03/23/2001 9:19:49 PM Eastern Standard Time
From: amos@nestgrp.com (Amos Heckendorf)
Sender: abrf-request@aecom.yu.edu (Association of Biomolecular Resource
Facilities)
To: abrf@aecom.yu.edu (Recipients of ABRF List)
>Dear ABRFers,
>
>Does anyone know of an academic or commercial lab that performs
>glucosamine analysis?
>
>Thanks,
>
>Dick
Dick
Shuster Labs 800-444-8705, but I do not know if they do outside work.
Alternatively, you can use our SCX column (p/n: P204SE0503 ) to do
glucosamines using ca. 20 -40% MeCN in a phosphate buffer at pH 3.0 .
One can get anomeric separations of the sugar at a higher pH, so pH
control is important.
Sincerely,
Amos Heckendorf
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