Mary,
we have made thiophosphorylated peptides using the post-synthesis
thiophosphorylation strategy. I am not aware of any vendor that sells
thiophosphorylated amino acid derivatives for peptide synthesis. Here are
some references.
Chemical Synthesis of pS,pT,pY:
Kitas, E. et al. (1994). Int. J. Pep. Prot. Res. 43, 146-153
Tegge, W. (1994). Int. J. Pep. Prot. Res. 43, 448-453
Mora, N. et al. (1995). Int. J. Pep. Prot. Res. 45, 53-63
Chemcial Synthesis of pH:
Lasker, M. et al. (1999). Prot. Sci. 8, 2177-2185
Pirrung, M.C. et al. (2000). J. Org. Chem. 65, 8448-8453
Enzymatic Synthesis:
Cassel, D. and Glaser, L. (1982). PNAS 79, 2231-2235
Li et al. (1988). Meth. in Enzymol. 159, 346-356
At 02:11 PM 3/22/01 -0800, you wrote:
>A researcher has just asked about the possibility of synthesizing a
>thiophosphorylated peptide. Either Ser or Thr, or possibly both. While we
>have made phosphopeptides by both incorporating a pre-phosphorylated
>derivative, or phosphorylating post-synthesis, we haven't done this.
>
>Does anyone have any ideas or suggestions? Can one buy a
>pre-thio-phosphorylated amino acid derivative?
>
>Thanks for your suggestions.
>
>
>
>Mary Kay Dolejsi, Ph.D.
>Manager, Biotechnology Lab
>mailstop- B1-080
>Fred Hutchinson Cancer Research Center
>1100 Fairview Ave N.
>PO Box 19024
>Seattle, WA 98109-1024
Chris (turck@itsa.ucsf.edu)
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