Laurey and Amanda,
Beware, ABI's kits while selling for a significant discount, were reported
earlier this year to contain R2B instead of their new product, R2C. This
was the information I was given out in Foster City in Feb. The R2C is the
same n-methylpiperidine as the R2B except that it is in butanol /
isopropanol instead of methanol. The chromatogram using R2C is supposed to
lack the contaminant peak which co-elutes with dptu allowing uses of dptu
as the reference peak once again. If you are satisfied with the R2B then
the kits may be the way to go but as the R2B/R2C costs are significant
(~$250/ bottle), if you are planning to use R2C, the kits may not be that
great of a bargain. It may be advantageous for people to call up ABI and
inquire / suggest that they switch to R2C in their kits.
David
At 11:48 AM 3/27/01 -0600, Laurey Steinke wrote:
>Hi Amanda,
>Here in the states about half of the protein sequencing products went off
>of the discounted list. The end result of this is also a steep price
>increase for us. I called ABI, and was given the number of the kit for
>protein sequencing. Apparently the chemicals are at a discount from the
>individual prices (I haven't added them up myself yet) AND our discount
>will apply to the kit price. S0....if they are right, the chemicals
>should be less expensive than before, BUT, will be less convenient (I think).
>
>The rationale behind this is the ever escalating costs of order
>processing, which are the same for a $100 bottle of reagent, as they are
>for a $2,000 kit. Now, they should have contacted us BEFORE they whacked
>our discounts or raised our prices, but (after I told them I did NOT like
>finding out about major price increases when I placed an order) they
>thought they would send out a letter. Better late than never.
>
>Soo....I will try the kit next time I order. If it is too inconvenient,
>or doesn't end up costing less, I too will be interested in the Sigma option!
>
>-laurey
>
>Weather inconsequential: Sunny day, warming slowly. Spring is late this
>year. None of my daffodils or hyacinths are blooming yet, but all are
>several inches tall. Busy as all get out!!!
>Laurey Steinke
>Protein Structure Core Facility
>University of Nebraska Medical Center
>Omaha Nebraska, 68198-4525
>
>Phone (402) 559-6647
>FAX (402) 559-6650
>http://www.unmc.edu/pscf/
>lsteinke@molbio.unmc.edu
>
>
David C. Chiara, M.D., Ph.D.
Research Associate
Department of Neurobiology
Harvard Medical School
220 Longwood Avenue
Boston, Ma. 02115
ph: (617) 432-1729
fax: (617) 734-7557
Email: David_Chiara@hms.harvard.edu
www.hms.harvard.edu/bss/neuro/cohen/
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