Hi Lynn,
For us, 10ng of genomic DNA in our PCR works fine.
Jennifer Brosius
-----Original Message-----
From: Bob Keefe [mailto:keefe@wadsworth.org]
Sent: Wednesday, March 28, 2001 8:56 AM
To: Recipients of ABRF List
Subject: Re: FA: DNA in PCR
Lynn,
We use 50-100 ng mouse DNA in 20 ul PCRs carried out in capped 96-well
plates with 0.2 ml well volumes. Do you use 5 ul PCR volumes in 384
plates? My initial concern using 5 ul volumes in 0.2 ml wells/tubes would
be possible adverse effects of volume changes (loss?) during thermocycling.
Bob Keefe
At 12:45 PM 3/27/2001 -0500, Lynn Petukhova wrote:
>
>Hello everyone:
>
>I have a quick question for people doing Genescan:
>
>How much DNA do you use in your PCR?
>
>We use 20ng in a 5uL reaction and I would like to reduce this. Thanks in
>advance for your feedback.
>
>Lynn
>
>
>Lynn Petukhova
>Assistant Director
>Genotyping Core Facility
>Starr Center for Human Genetics
>The Rockefeller University
Robert G. Keefe, Ph.D.
Wadsworth Center/NYS Dept. of Health
Genomics Core Facility
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