If you need to do high sensitivity work, some sequencer reagents are not
that easy to make correctly. What people don't seem to understand is that you
not only need a reliable source for the components, but you have to QA the
stuff every time you get a new bottle. Think about how many times the
sequencer reagent vendors have screwed things up. And they do it for a
living and have a lot more analytical support than you or I do. Did you get
a bad bottle of ethyl acetate in the past year? No matter who you bought
"S2B" from, I'll bet you got at least one bad one.
And the time commitment to making your own reagents isn't as trivial as
you think. Yeah, you can make your own S4B and R4A (unless you have the
"brown" TFA), but wait until you try to find a good source for chemicals like
PITC, TMA and NMP. You'll find yourself reading Zubrick's "The Organic Chem
Lab Survival Manual" trying to remember how to do recrystallizations and
vacuum distillations.
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