This is all a costing discussion and I am certain that those who are getting
involved in this 'home-made' process are fully aware of these simple
concerns. Rest assured none of us truly believe that anything, except
perhaps drinking beer, is simple!!!
----- Original Message -----
From: <ST51583744750@aol.com>
To: "Recipients of ABRF List" <abrf@aecom.yu.edu>
Sent: Thursday, March 29, 2001 4:17 PM
Subject: Re: ProtSeq Reagents
> If you need to do high sensitivity work, some sequencer reagents are
not
> that easy to make correctly. What people don't seem to understand is that
you
> not only need a reliable source for the components, but you have to QA the
> stuff every time you get a new bottle. Think about how many times the
> sequencer reagent vendors have screwed things up. And they do it for a
> living and have a lot more analytical support than you or I do. Did you
get
> a bad bottle of ethyl acetate in the past year? No matter who you bought
> "S2B" from, I'll bet you got at least one bad one.
>
> And the time commitment to making your own reagents isn't as trivial
as
> you think. Yeah, you can make your own S4B and R4A (unless you have the
> "brown" TFA), but wait until you try to find a good source for chemicals
like
> PITC, TMA and NMP. You'll find yourself reading Zubrick's "The Organic
Chem
> Lab Survival Manual" trying to remember how to do recrystallizations and
> vacuum distillations.
>
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