Does anyone know of a method to determine protein concentration in materials
that are chelated to heavy metals? Colormetric methods have been tried, but
exhibit high imprecision, and I have concerns about the accuracy of results
coming from a standard curve made with protein not chelated to metal. Amino
acid analysis was attempted but the heavy metal appeared to interfere with
the pre-column derivatization. An extinction coefficient is not yet
determined, so UV is not very useful yet. Ideas?
Nadine Ritter, Ph.D.
Director, Analytical Services
BioReliance Corp.
nritter@bioreliance.com
301-610-2587
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