You make some very good points, but most of us would be more willing to pay
attention to what you say, if we knew who you were. Could you please include
some kind of "signature" with contact information next time ?
BTW: If you really need anonymity, there is always the Ombudsman
Thanks for pointing out the QC issue.
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
ST51583744750@aol.com wrote:
> If you need to do high sensitivity work, some sequencer reagents are not
> that easy to make correctly. What people don't seem to understand is that you
> not only need a reliable source for the components, but you have to QA the
> stuff every time you get a new bottle. Think about how many times the
> sequencer reagent vendors have screwed things up. And they do it for a
> living and have a lot more analytical support than you or I do. Did you get
> a bad bottle of ethyl acetate in the past year? No matter who you bought
> "S2B" from, I'll bet you got at least one bad one.
>
> And the time commitment to making your own reagents isn't as trivial as
> you think. Yeah, you can make your own S4B and R4A (unless you have the
> "brown" TFA), but wait until you try to find a good source for chemicals like
> PITC, TMA and NMP. You'll find yourself reading Zubrick's "The Organic Chem
> Lab Survival Manual" trying to remember how to do recrystallizations and
> vacuum distillations.
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
If I have seen farther than others, it is because I was standing on the
shoulders of giants.--Isaac Newton
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