Thank you to everyone who responded to my question. Here is a summary of
responses:
50-100 ng in 20uL reaction (2.5-5.0 ng/uL) for genescan
12.5 ng in 5uL reaction (2.5 ng/uL) for genescan
10 ng in 10uL reaction (1.0 ng/uL) for unspecified application
40-60 ng in 20uL reaction (2.0-3.0 ng/uL) for genescan
10 ng in 50uL reaction (0.2 ng/uL) for pyrosequencing
A few people had asked about the plates and seals we use for 5uL reactions.
We use 384 well PCR plates from AB, their clear adhesive seals, and 9700s.
Lynn Petukhova
Assistant Director
Genotyping Core Facility
Starr Center for Human Genetics
The Rockefeller University
1230 York Avenue, Box 241
New York, NY 10021
phone: (212) 327-7181
fax: (212) 327-7182
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