Dear ABRFers:
The Nucleic Acids Research Group (NARG) of the ABRF invites you to visit
our new site where the data of the 2001 oligo quality control (QC) study is
ready for review/download. We like to acknowledge the contributions of the
participants.
ftp://ftp.fccc.edu/yeung/outgoing/
You can start at the poster, or at the "readme first" file.
The site consists of the quality control of about 31 DNA synthesizers. The
744 QC chromatograms are categorized by six methods of analysis, and again
according to the laboratory submission code numbers. Also included are the
UV traces of all the oligos, and the QC presentations at ABRF2001. We use
homopolymers to evaluate the synthesis efficiency of each of the four
nucleotides for each DNA synthesizer. You will see that even instruments
that make good looking primers may have non-optimum synthesis of one or
more of the four nucleotides, therefore unlikely to make good long oligos
or custom oligos.
For those who are new to this study, you can QC your own oligos this way,
and compare your oligo quality with others in this database. You can
choose the QC method that works best for you. Routine QC will lead to DNA
synthesizers that make better oligos, sometimes even cheaper and faster.
We know you can do it, and we stand ready to help. Please get in touch
with us.
Sincerely,
Tony Yeung (Chair)
Martha Gunthorpe (Former Chair)
Jay Fox
Brian P Holloway
Kathryn Lilley
Kathleen Mills
Stephen Scaringe
Scottie Adams (FARG ad hoc)
Ted Thannhauser (DSRG ad hoc and EB ad hoc)
The ABRF reserves the copyright to the contents of this ftp site.
Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Fox Chase Cancer Center
7701 Burholme Avenue, Philadelphia, PA 19111
voice: 215-728-2488
fax: 215-728-3647
email: AT_Yeung@fccc.edu
http://www.fccc.edu/research/labs/yeung/
This archive was generated by hypermail 2b29 : Tue May 01 2001 - 14:07:05 EDT