Scottie,
The gels we get are on plastic, hopefully they won't melt!
Also, although it is probably obvious to you, for others I failed to mention that these are the gels with urea.
Todd
>>> Scottie Adams <sadams@northnet.org> 04/06 10:20 AM >>>
Todd,
It is still on the glass plate. However, it is important to wash
the urea out of the gel before drying or the glass plate will break (or so
they say).
Yes, please let me know how it owrks for you. Thanks
S.
At 10:09 AM -0400 4/6/01, Todd Skaar wrote:
>Hi Scottie,
>
>Thanks for the info, I'll give it a try and let you know how it works.
>
>What do you put the gel on while you are drying it (e.g. filter paper,
>plastic support of some type, stretcher ?)
>
>Todd
>
>
>
>>>> Scottie Adams <sadams@northnet.org> 04/06 10:04 AM >>>
>Hi Todd
>
> Thanks for your suggestion. We will check it out.
>
>As for drying in the microwave, this works for us.
>
>Soak gels in water for 15 minutes
>Dry gels in microwave for 2.5 minutes at 50% power
>
>Directions say time should be determined using 30 sec cycles until dry. A
>dry gel appears as a thin layer and is not sticky. Be sure to cool before
>exposing to phosphorimager or film. Also be sure that any attachment strips
>are removed first.
>Directions say 20 minutes in an 80C oven also works but we have never done
>this.
>
> I'm not sure if this will work for other gel types (since I don't know
>exactly what is in this gel??), but it is so quick and easy it is worth a
>try.
>
>Scottie
>
>
>
>
>At 9:45 AM -0400 4/6/01, Todd Skaar wrote:
>>We routinely use the Novex 6%TBE gels for RNase protection assays with
>>good results. We dry them on a regular gel dryer. This may be an
>>alternative, although maybe not quite as fast.
>>
>>I would be interested in your microwave gel drying protocol (i.e. any
>>extra apparatus required, what types of gels can be dried this way,
>>reference, etc.)
>>
>>Thanks,
>>Todd
>>
>>Todd Skaar, Ph.D.
>>Lombardi Cancer Center
>>Director of Macromolecular Analysis Shared Resource
>>E401, The Research Building
>>Pager# (202) 490-2454
>>Phone: (202) 687-6466
>>
>>
>>>>> Scottie Adams <sadams@northnet.org> 04/05 4:25 PM >>>
>>Hello everyone
>>
>>While we are on the subject of discontinued gels, perhaps there is some
>>help for me also.
>>We are currently using An Invitrogen,(actually Novex) gel called the
>>Quickpoint Rapid Nucleic Acid Separation System.
>>
>>We use them for RNase protection assays and have been quite pleased with
>>them as far as ease of use, separation, etc. A gel can be run in 20
>>minutes and dried in a microwave, making them very quick and efficient.
>>
>>
>>Recently we tried to order more, and we have been informed that they will
>>no longer make them. This puts a serious crimp in our plans.
>>Invitrogen was kind enough to send us their remaining stock- so we are not
>>desperate yet, but we need to resolve the problem in the next few months.
>>If anyone has any suggestions, I would welcome them. If a gel expert has
>>any idea of the compostion of these gels , we would be glad to make our
>>own. They are a neutral pH, denaturing polyacrylamide gel. We are very
>>spoiled and would not like to go back to using larger gels. Or if anyone
>>has any of these gels that they would part with- we'll take them.
>>I suppose we can experiment on our own- but time is at a premium and so I
>>will try this way first. :-).
>>
>>Thanks
>>
>>Scottie
>>
>>Scottie Adams aka Pamela Scott Adams
>>
>>Manager
>>Molecular Biology Core Facility
>>Trudeau Institute
>>100 Algonquin Avenue
>>Saranac Lake, NY 12983
>>Phone: 518-891-3080, Ext. 115
>>Fax: 518-891-5126
>>Email: sadams@northnet.org
>>sadams@trudeauinstitute.org
>>http://www.trudeauinstitute.org
>
>
>Scottie Adams aka Pamela Scott Adams
>
>Manager
>Molecular Biology Core Facility
>Trudeau Institute
>100 Algonquin Avenue
>Saranac Lake, NY 12983
>Phone: 518-891-3080, Ext. 115
>Fax: 518-891-5126
>Email: sadams@northnet.org
>sadams@trudeauinstitute.org
>http://www.trudeauinstitute.org
Scottie Adams aka Pamela Scott Adams
Manager
Molecular Biology Core Facility
Trudeau Institute
100 Algonquin Avenue
Saranac Lake, NY 12983
Phone: 518-891-3080, Ext. 115
Fax: 518-891-5126
Email: sadams@northnet.org
sadams@trudeauinstitute.org
http://www.trudeauinstitute.org
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