Stephanie,
It could very possible remove a significant portion of the Fmoc protection.
Stay away from primary amines. I suggest using DIEA/DMF, as the
diisopropylethylamine does not participate aggressively in the
beta-elimination route for Fmoc removal.
Best regards, Dick
Stephanie Michaud <Michaud@adherex.com>@aecom.yu.edu> on 04/06/2001
12:24:56 PM
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Subject: FMOC removal?
Dear All,
I would like to react FMOC-Cys-NH2 via the sulfhydryl group with an
N-bromoacetylated peptide to form a thioether. Will using TRIS as base (in
DMF) cause significant removal of the FMOC group? Thanking you in advance.
Stephanie
Stephanie Michaud, Ph. D.
Project Leader
Adherex Technologies Inc.
ph 613.738.8000 ext227
fx 613.738.9060
email michaud@adherex.com
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