The best way is the way you can do well and relate to.
For me, it is either partial nuclease degradation followed by mass
spectrometry, or
P32 labeling followed by chemical DNA sequencing.
reference:
Yeung, A. T., Dinehart, W. J., and Jones, B. K. Modifications of guanine
residues during oligonucleotide synthesis. Nucleic Acids Res 16:45394554,
1988.
Hope this is useful.
Tony
At 10:22 AM 4/10/2001 -0500, rpershad@mail.mdanderson.org wrote:
>
>
>I was wondering what is the best way to sequence short oligos. Responses
>greatly
>appreciated.
> Thanks.
>
>
************************************
Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave., Philadelphia, PA 19111
Voice: 215-728-2488 FAX: 215-728-3647 email: AT_Yeung@FCCC.edu
Public Key: D994 4658 6AE2 12A6 A538 D18C 461F 5ED2 21C5 2AFF
http://www.fccc.edu/research/labs/yeung/
************************************
This archive was generated by hypermail 2b29 : Tue May 01 2001 - 14:07:05 EDT