Susana,
The main reason that we switched from R2 (12.5% trimethylamine) to R2B was
the odor. Whenever I changed the R2, the whole floor knew it and
complained and the odor lingered for hours. Unfortunately, if your
programs are optimized for R2 you may need to contact ABI for adjustments
for switching to the R2B / R2C reagent. I believe that ABI has increased
the cost of R2 in an attempt to phase it out.
R2B and R2C are both N-methylpiperidine with different solvents. The
problem with using R2B is that an additional peak elutes next to the dptu
peak and if one uses the dptu peak as their reference peak, the 610
integration fails. Supposedly, R2C lacks that peak allowing the dptu peak
to be used as a reference peak. Both are quite expensive ($ 250) although
I believe that a bottle of R2B last considerably longer than a bottle of R2
used to last (ABI could probably give #s).
David
David C. Chiara, M.D., Ph.D.
Research Associate
Department of Neurobiology
Harvard Medical School
220 Longwood Avenue
Boston, Ma. 02115
ph: (617) 432-1729
fax: (617) 734-7557
Email: David_Chiara@hms.harvard.edu
www.hms.harvard.edu/bss/neuro/cohen/
This archive was generated by hypermail 2b29 : Tue May 01 2001 - 14:07:05 EDT