Hello all:
I am having curious problems with my 3700 and was wondering if anyone else
has experienced the same things.
I am in a core facility, so we run samples for many different people on the
same 96 well plate. I noticed on a recent plate that the contig maps were
showing mismatches. It seems that samples the software is calling one thing
are actually something else. In other words, the sequence from what it
calls well A2 is actually from another well, but I have no idea which well.
I have no way of knowing or figuring out what should be where.
We confirmed that there is a problem by taking the same plate to another
3700 in another facility, where it's results were fine and everything made
sense and matched up.
I then ran two staggered Pgem control plates. One plate had pgem in rows A,
C, E and G with the other rows blank. The second plate had pgem in 1, 3, 5,
7, 9, and 11, with the others blank. This plate also confirms that there is
a problem somewhere because there was sequence in places where there
shouldn't have been and vice-versa. It doesn't seem to be a problem with
the actual loading, based on the results from these staggered plates. It
just makes no sense at all!
I currently have the 1-800 ABI Tech support looking at the problem, but
have not heard back from them. Has anyone else experienced this strange,
aggravating problem??
Many thanks,
Peggy
Peggy Grow
Biopolymers Facility
Howard Hughes Medical Institute
Harvard Medical School
Warren Alpert Building, rm. 341
200 Longwood Avenue
Boston, Massachusetts 02115
phone: 617/432-7481
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