RE: DNA sequence turn around time

From: Karen M. Clare (kmclare@RICETEC.COM)
Date: Thu Apr 19 2001 - 09:10:50 EDT


Replying to your comment: "If you have a 3100 that can only do 96 samples
in 24 hours you have to have a big investment in equipment to equal the
output of a 377 that can run 4 or 5 gels a day at the 4x speed". We have
a 3100 with 384 well plate holder. We can run 576 samples in 24 hours. We
need only load the 3100 once a day. We retired our 377 because it was labor
intensive.

                -----Original Message-----
                From: Hills, Harold [mailto:Harold.Hills@umassmed.edu]
                Sent: Wednesday, April 18, 2001 11:22 PM
                To: Recipients of ABRF List
                Cc: 'wfehr@iastate.edu'
                Subject: DNA sequence turn around time

                Next day service is not out of line for on campus users. We
accomplished
                this for many years at Iowa State. It makes sense to run a
2nd or even a
                3rd shift, operating round the clock to make best use of
your instruments.
                Of course with the 3100 or 3700 you can set them and forget
them and they
                work through the night. If you have a 3100 that can only
do 96 samples in
                24 hours you have to have a big investment in equipment to
equal the output
                of a 377 that can run 4 or 5 gels a day at the 4x speed.
It may not be the
                best use of huge amounts of money to have it tied up in
instruments which
                become obsolete if one must always have the absolute latest
model equipment.

                Hal

                Harold G. Hills, Ph.D.
                Department of Cell Biology, S3-308
                Harold.Hills@umassmed.edu
                University of Massachusetts Medical School 508
856-2541
                55 Lake Avenue North
                Worcester, MA 01655

                Information on trouble shooting 377 Sequences at URL below.

        
http://www.biotech.iastate.edu/facilities/DSSF/ABRF/default.html



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