Hello all:
Thanks again for all of your input on my strange problems with loading on
the 3700. It turns out that Brain Coullahan had the correct answer in that
our autoloader tips were bent! Thank you, Brian, for suggesting that
possibility and helping to solve our problems. We also replaced the array,
so it could have conceivably been a combination of the two events causing
the loading mismatches. I would encourage all 3700 users to check your
autoloader tips frequently by unscrewing them and rolling them to see if
they are bent. Mine weren't bent enough to notice while in the machine, but
it was very obvious once I took them off and rolled them across the bench!
By the way, ABI Tech Support, while very helpful, told me that there was no
way that the tips could be bent or be the cause of the problem!
Have any of you changed the cuvette or run temperatures in the run module?
What effect did it have on your signal strengths? Do you think that
injection times have an effect on signal strengths? Mine are not as strong
as I would like and am wondering what I could alter to increase them.
Thanks,
Peggy
Peggy Grow
Biopolymers Facility
Howard Hughes Medical Institute
Harvard Medical School
Warren Alpert Building, rm. 341
200 Longwood Avenue
Boston, Massachusetts 02115
phone: 617/432-7481
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