Lisa,
I think one of the problems mentioned by Lekha is that the default
chromatogram file format for the MegaBACE (.esd) is not read by commonly
used software like editview, so clients cannot see their data like they can
with the ABI systems. However, the MegaBACE collection software can be
configured to create an ABI formatted file that is read. This works pretty
well, except that any data in comments or other fields are not transferred
from the esd to the abi file, so information can be lost. Also the esd
files have funny start and end times. We see that in a run of 96, some of
the files can have run end times a second earlier than the others. Not a
big deal, unless you need to group data by run start and end times.
Cheers,
Todd
Todd M. Smith, Ph.D.
President
Geospiza, Inc.
www.geospiza.com
-----Original Message-----
From: Association of Biomolecular Resource Facilities
[mailto:abrf-request@aecom.yu.edu]On Behalf Of Sreelekha Devarayalu
Sent: Thursday, April 19, 2001 10:21 AM
To: Recipients of ABRF List
Cc: Recipients of ABRF List
Subject: Re: DNASeq:I need to talk to a MEGABASE user
Lisa ,
For a group networked into the Megabace , it is a great instrument .
However , if yours is a multiuser core lab that needs to *dispense* data ,
the Megabace is nothing short of a nightmare !
Lekha
Sreelekha Devarayalu
Research Scientist
Biochemistry DNA Sequencing Facility
University of Washington
Seattle
(206) 616-7491
On Wed, 18 Apr 2001, lisa bibbs wrote:
> Does anyone have one? How do you like it? Tell me about
> reliability, ease of operation, .....
>
> Thanks in advance,
> Lisa
> Lisa Bibbs, Ph.D.
> 10550 N. Torrey Pines Rd.
> SP3
> La Jolla, CA 92039
>
> http://corefac.scripps.edu
> 858 784-9941
>
>
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