Susan
We are a core facility and have a couple of 377's, a 310 and a CEQ2000XL.
Though there is the time advantage to sequencing with the CEQ2000, there really
is no comparison when it comes to read length. We have been consistently
getting 0.8-1 kb read lengths on the 377 and barely reading through to 600 bases
on the CEQ2000.
Their tech support was really inferior until I was able to contact a certain
tech support specialist who actually knew what he was talking about. His name
is John Stryker (and is located in San Fran) with email jmstryker@beckman.com -
I'm sure he'll be willing to help with troubleshooting. There are definite
limitations with the CEQ2000 right now and hopefully their software engineers
will address these with their next upgrade. We have had issues with the
analysis software being unable to analyse short amplicons defaulting to a "Not
enough peaks in the data file" error. Seems that the analysis software can
handle only minimum of 150-200 bases or so.
Claude Ouellette
DNA Core Facility
Canadian Science Centre for Human and Animal Health
1015 Arlington St., Suite H3380
Winnipeg, MB R3E 3R2
Ph: 204-789-6035
Fax: 204-789-2018
EMail: claude_ouellette@hc-sc.gc.ca
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc: (bcc: Claude Ouellette/HC-SC/GC/CA)
Subject: DNA sequencing
Hi,
I was wondering if anyone out there is using the Beckman CEQ2000 with the
XL upgrade that would like to communicate regarding trouble shooting, data
handling, etc.?
Susan San Francisco
Susan San Francisco
Biotechnology Core Facility
Center for Biotechnology and Genomics
Texas Tech University
Lubbock, TX 79409
Phone: 806-742-0942
FAX: 806-742-2025
susan.san-francisco@ttacs.ttu.edu
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