> By fragments, do you mean MS/MS fragments? Were the main peaks the
> predicted mass for the peptides?
>
> Katheryn Resing
Dear Katheryn, Thank you for your answer. By fragments I means fragment
obtain after enzymatic degradation. The enzyme used is not specific enzyme
as trypsin, and I tried to define the specificity of cleavage of this enzyme
on a synthetic peptide. I obtain some degradation products and usually I can
easily with the mass identified the site of the cleavage. In some case, I
found additional minor peaks and this peak is always at -60.7 Da from the
main degradation products.
Luc
>> Hi
>>
>> I have a synthetic peptide of 28 AA's. I run different cleavage and then
>> separation of the resulting fragments on reverse phase HPLC in H2O/CH3CN
>> gradient including 0.1%TFA. Then the fractions were lyophilized.
>> When I run the mass spect, I found some fragments showing a doublet at mass N
>> for the major peaks and N -60.7 for the minor peaks. What could this -60.7
>> be?
>>
>> Does anyone have any explanation about this -60.7?
>>
>>
>> Thanks in advance
>>
>> Luc CAMOIN
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