peptide purification

From: gillespiep (gillespiep@uthscsa.edu)
Date: Wed May 02 2001 - 16:13:54 EDT


<x-charset ISO-8859-1>i have a peptide with the sequence

EERRKEKHRKMEEEREEMRQTIRDKYGLKK

i've had successful synthesis of the peptide but upon purification i was
unable to pull it apart from a fragmented version. i have changed the hplc
gradient to almost flat and still these two elute at the same time within the
same fractions. any suggestions?

Paula Gillespie
Research Assistant
University of Texas Health Science Center
San Antonio, TX 78240

</x-charset>



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