Hi to all,
Has anyone tried doing in-gel digest from protein separated only in the first dimension of an IPG?
Even if you haven't, I would be glad to hear your thoughts on how to approach developing a protocol
for one. We want to mass map a band of interest on a Coomassie stained IEF strip.
Would the protein recovery be low due to loss during pre-digestion washes?
Should we cut through the backing and leave it in the reaction mix or scrape the gel off and deal
with the gel pieces?
Do you foresee any problems during reduction/alkylation that may arise due to the presence of the
immobilized ampholines?
Linda
Big Apple Minute: It's summer in spring here. Time to be out enjoying the lilacs and cherry
blossoms. I heard that the California late fall rains have turned the desert into a bloom of the
century and wish I could see it too!
Linda Siconolfi-Baez
Laboratory for Macromolecular Analysis
Albert Einstein College of Medicine
Ullman 405
1300 Morris Park Avenue
Bronx, N.Y.10461
718-430-2864
lsiconol@aecom.yu.edu
http://www.bioc.aecom.yu.edu/labs/angellab/
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