I suspect that the problem might be fmoc deprotection in additiion/ instead
of a coupling problem.
Try 2%DBU/DMF as the deFmoc-ing reagent and see how it goes
Denis Scanlon
AuspepPty Ltd
At 06:04 AM 3/05/01 , gillespiep wrote:
>i've tried unsuccessfully several times to synthesize a very hydrophobic
>peptide with the
>sequence:
>
>CTLVGIIVGVLLAIGF
>
>i have tried straight synthesis with doubling couplings between each amino
>acid. that didn't
>work. i was getting what appeared to be a break between the I and V. i
next
>tried to extra
>couple between those two amino acids and still got the break. my mass was
>889. next
>synthesis i stopped after this point to check the synthesis and successfully
>obtained the correct
>mass for IVGVLLAIGF. after continuing the rest of the synthesis i again
>obtained the mass of
>889. does someone have any suggestions on a way to synthesize this peptide
>using Fmoc
>chemistry? can anyone explain why i seemed to have lost my I on the third
>try.
>
Auspep Pty Ltd
115 Ireland St
West Melbourne
Australia 3003
This archive was generated by hypermail 2b29 : Wed Nov 21 2001 - 14:16:28 EST