Try cation IX at pH5.5 with a NaCl gradient to 1M and then desalt on RP HPLC
Alternatively RP HPLC prep at pH5.5-6.5 in sodium phosphate followed by a
TFA buffer desalt ( I am assuming that you only used TFA buffer for the
first attempt)
regards
Denis Scanlon
Auspep Pty Ltd
At 06:13 AM 3/05/01 , gillespiep wrote:
>i have a peptide with the sequence
>
>EERRKEKHRKMEEEREEMRQTIRDKYGLKK
>
>i've had successful synthesis of the peptide but upon purification i was
>unable to pull it apart from a fragmented version. i have changed the hplc
>gradient to almost flat and still these two elute at the same time within
the
>same fractions. any suggestions?
>
>Paula Gillespie
>Research Assistant
>University of Texas Health Science Center
>San Antonio, TX 78240
>
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115 Ireland St
West Melbourne
Australia 3003
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