Dear Dennis
you should be able to run the cellular extract on a SDS-PAGE and to
identify the region where the two proteins should migrate by western blot.
A second lane of the gel can then be blotted on a PVDF membrane and the
protein band sequenced. The sequencer will be able to tell you whether the
band contains one or both proteins and even their relative abundance.
Best regards
Prof. Piero Pucci
At 09.24 15/05/01 -0700, you wrote:
> I have a problem and I thought I might be able to find some help
>in this discussion group. I need a way to take whole cells, break
>them open, and then see if both proteins are being translated. Thanks,
>Dennis.
>
>
Prof. Piero Pucci
Dipartimento di Chimica Organica e Biochimica
Complesso Universitario Monte S. Angelo
via Cinthia - 80134 Napoli
Tel.: 081-674318 - Fax: 081-674313
e
Centro Internazionale Servizi di Spettrometria di Massa
via Pansini 5 - 80131 Napoli
Tel.: 081-5465136 - Fax: 081-5465484
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