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<div>Hi Dennis,</div>
<div>I'd try to attach 2 different epitope tags, say myc and HA. Then
a Western should be fine.</div>
<div><br></div>
<div>Thomas</div>
<div><br></div>
<div><br></div>
<blockquote type="cite" cite><font face="Arial" size="-1">Hi
everyone, </font><br>
</blockquote>
<blockquote type="cite" cite><font face="Arial" size="-1">I have a
problem and I thought I might be able to find some help in this
discussion group.</font><br>
</blockquote>
<blockquote type="cite" cite><font face="Arial" size="-1">I am
co-expressing two proteins in CHO cells and then trying to detect if
one or both of them are expressed. The problem is that the two
proteins are identical except for short peptide sequence at the
C-terminus. I have antibodies that react with both fragments but
not with each individually. The whole proteins should be
approximately 50 and 53 kDa in size so separation on Western would be
difficult. </font><br>
</blockquote>
<blockquote type="cite" cite><font face="Arial" size="-1">I need a way
to take whole cells, break them open, and then see if both proteins
are being translated.</font><br>
</blockquote>
<blockquote type="cite" cite><font face="Arial"
size="-1">Thanks,</font><br>
</blockquote>
<blockquote type="cite" cite><font face="Arial"
size="-1">Dennis.</font></blockquote>
<div><br></div>
<div><tt><font color="#000000">-- <br>
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This archive was generated by hypermail 2b29
: Wed Nov 21 2001 - 14:16:55 EST
Dr. Thomas Weber<br>
Institute for Gene Therapy and Molecular Medicine<br>
Box 1496<br>
Mount Sinai School of Medicine<br>
1425 Madison Avenue<br>
New York, NY 10029-6574<br>
United States of America<br>
</font></tt></div>
<div><tt><font color="#000000">Phone (office): (212) 659
8293</font></tt></div>
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659 8299</font></tt></div>
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2437</font></tt></div>
<div><tt><font color="#000000">e-mail:
thomas.weber@mssm.edu</font></tt></div>
<div><tt><font
color="#000000">http://www.mssm.edu/genetherapy/weber/></tt></div
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