DNA Sequencing problem

From: Debbie Bradley (dbradley@molbio.med.miami.edu)
Date: Wed May 16 2001 - 10:07:21 EDT

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<x-charset iso-8859-1>Hope someone can help us. We have been seeing an unusual blue haze at the
beginning of two different clients samples. It seems to be specific to
certain primers which they are using. Same DNA run with other primers does
not produce the blue. Clients say that the primers are diluted in water
only. We run the samples on an ABI 377 with the Big Dye chemistry. Has
anyone seen this before?

Debbie Bradley/ DNA Core Lab
Univ. of Miami School of Medicine
Gautier 305
1011 NW 15th St.
Miami, FL 33136

305-243-6998
305-243-2058 Fax

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