<x-charset iso-8859-1>Dear Isabelle:
1. Methanol is not usually used for couplings, though it may not interfere.
But I know that isopropanol does NOT interfere with couplings - it is less
reactive because it is a secondary alcohol.
2. References will come later.
NLB.
At 10:52 AM 5/16/01 -0400, you wrote:
>Dear Dr Benoiton,
>
>I was just trying to find your email address when I received your email!
>Thank you for all this information!
>
>I've tried to solubilize the Fmoc-dimethyl-lysine in DMSO and in CH2Cl2 and
>it didn't work well, but in methanol, it's highly soluble. Is it a good
>idea to solubilize the amino acid in methanol and then do the usual
>coupling reaction manually with HBTU/HOBT in DMFand DIEA in NMP. Would the
>methanol interfere during the reaction ?
>
>I'm very interested to have some references about the hydrogenation process
>(or other reference that you think might be interesting in my case...).
>
>Best regards,
>isabelle
>
>Isabelle Kelly MSc.
>Service Protéomique de l'Est du Québec,
>CHUQ Pavillon CHUL,
>2705, Boul.Laurier,
>Ste-Foy, Québec,
>G1V 4G2
>
>Tel. 418-656-4141 ext. 7088
>Fax. 418-654-2159
>http://www.crchul.ulaval.ca/crchul/en/serv/Default.htm
>
N. Leo Benoiton Professor Emeritus of Biochemistry
Faculty of Medicine
University of Ottawa, Tel: 613 562 5800 ext. 8216
451 Smyth Road, Fax: 613 562 5440
Ottawa, Ontario, Canada K1H 8M5 eMail: benoiton@uottawa.ca
</x-charset>
This archive was generated by hypermail 2b29 : Wed Nov 21 2001 - 14:16:55 EST