Re: Met-O sequencing

From: Daniel Wellner (dwelln@med.cornell.edu)
Date: Mon May 21 2001 - 13:46:00 EDT

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I tried it under the standard conditions I use for sequencing in the ABI
470 and the methionine sulfoxide did not survive. It appeared as
PTH-methionine. If someone knows of a method that works, I am also
interested.

Daniel

At 2:25 PM -0400 5/18/01, Ting Wong wrote:
>Hi:
>
>I wonder if Met-sulfoxide can survive the Edman condition used in sequencer.
>If so, is there any standard available commercially to track the position on
>chromatogram.
>
>Thanks for your attention.
>
>Ting Wong
>Scientist
>Biomira Inc.
>Edmonton

Daniel Wellner, Ph.D.
Department of Biochemistry
Weill Medical College of Cornell University
New York, NY 10021

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