Ting et al.
In my experience, Met(O) always reverts to Met during protein sequence
analysis. It should be feasible to assign Met(O) sites based on their
resistance to CNBr cleavage (i.e., after CNBr treatment, sequence the mixture
and look for the absence of an internal sequence that starts after Met; this
analysis works best if you have a non-oxidized control).
Reference:
Shechter et al. (1975) Biochemistry 14 (20), 4497-
- Reed Harris
Genentech, Inc.
1 DNA Way
South San Francisco, CA 94080
Ting Wong wrote:
> Hi:
>
> I wonder if Met-sulfoxide can survive the Edman condition used in sequencer.
> If so, is there any standard available commercially to track the position on
> chromatogram.
>
> Thanks for your attention.
>
> Ting Wong
> Scientist
> Biomira Inc.
> Edmonton
This archive was generated by hypermail 2b29 : Wed Nov 21 2001 - 14:16:55 EST