As far as I understand,the sequencing conditions are reducing,to prevent
desulfurization,and ,the sulfoxide(SO),reverts to methionine.Methionine
sulfone(SO2) gives a stable PTH.
Fulvio Perini
UNMC
986805 Nebraska Med.Cntr.
Omaha,NE 68198-6805
"Daniel Wellner"
<dwelln@med.corne To: Recipients of ABRF List
ll.edu> <abrf@aecom.yu.edu>
Sent by: cc:
Association of Subject: Re: Met-O sequencing
Biomolecular
Resource
Facilities
<abrf-request@aec
om.yu.edu>
05/21/2001 12:46
PM
I tried it under the standard conditions I use for sequencing in the ABI
470 and the methionine sulfoxide did not survive. It appeared as
PTH-methionine. If someone knows of a method that works, I am also
interested.
Daniel
At 2:25 PM -0400 5/18/01, Ting Wong wrote:
>Hi:
>
>I wonder if Met-sulfoxide can survive the Edman condition used in
sequencer.
>If so, is there any standard available commercially to track the position
on
>chromatogram.
>
>Thanks for your attention.
>
>Ting Wong
>Scientist
>Biomira Inc.
>Edmonton
Daniel Wellner, Ph.D.
Department of Biochemistry
Weill Medical College of Cornell University
New York, NY 10021
This archive was generated by hypermail 2b29 : Wed Nov 21 2001 - 14:16:55 EST