Hi,neighbor! Well,I did not see any replies,and I never ran thyroxine,and,I
do not use ion exchange,but I know something,although you may have drawn
your own conclusions.
I have run a synthetic peptide with diiodotyrosine,and all I saw was
tyrosine,in rather low amounts.I got the amino acid,after the fact,and
using vapor phase hydrolysis,I could not see anything.So,I went to plan
B,which is to extend the program from up to 38%B to 50%B,same rate,just
longer gradient.And,sure enough I saw a nice peak about 52min(I shutdown
the integrator at 50min.After acid hydrolysis,that peak was completely
gone,and tyrosine appeared.
The two iodines cause a 15 min shift. So,if we shift to thyroxine,I would
expect that it be deiodinated,but the ether bond be
tween phenol moieties,be stable.If not you should see some tyrosine.So,acid
hydrolysis would tear up your molecule.
Anyway,you know where I am,in case you want me to try anything.Oh,yes,I am
using AccQTag chemistry.
Fulvio Perini
UNMC
986805 Nebraska Med.Cntr.
Omaha,NE 68198-6805
Laurey Steinke
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Hello all and sundry!
I have had a request to run amino acid analysis and identify
thyroxine. (We use vapor phase HCl hydrolysis and post column
ninhydrin derivitazation on a Beckman 6300). Does the thyroxine
break down in the hydrolysis? Does anyone (Lowell?) know where it
comes out on the chromatogram?
Thanks!
-laurey
Weather Inconsequential: Well, okay, it's not weather. I let my
younger son get a puppy. This is our fourth animal, third dog. (A
large golden retriever that looks to my husband, a collie-german
shepherd mix whose heart belongs to my older son, and I have a cat).
The puppy is very young, 2 months, and very cute, and has just quit
whining at night (Yeah!!!)
Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525
Phone (402) 559-6647
FAX (402) 559-6650
http://www.unmc.edu/pscf/
lsteinke@molbio.unmc.edu
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