<x-charset ISO-8859-1>I have a peptide that is very hydrophobic that through trial after trial I was
finally successful in synthesizing but now have the tough task of purifying.
It was suggested that I check with the discussion group peptide professionals
and see what suggestions you have on purification. I am checking to see
anyone's experience with a CN or C8 column or using pyridine/propanol on
RP-HPLC. During previous purifications the peptide was lost on the C18
column. If anyone suggests using the above procedures or has one that has
worked better for them then any information is very appreciated. Thanks in
advance.
SEQUENCE: TLVGIIVGVLLAIGF also made with C at N terminus.
Paula Gillespie
Research Assistant
University of Texas Health Science Center
San Antonio, TX 78240
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