"Recipients of ABRF List" <abrf@aecom.yu.edu>
Subject: Re: peptide purification
Date: Tue, 22 May 2001 19:11:12 -0700
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Paula-
from
http://www.nestgrp.com/protocols/polylc/polysulfoethyl.shtml
For more strongly hydrophobic peptides, a non-ionic surfactant (at a concentration below
its CMC) and/or acetonitrile or n-propanol as mobile phase modifiers, can substantially
improve resolution and recovery over conventional reverse phase methods.
and
http://www.nestgrp.com/pdf/Pp1/HILIC_example.pdf
Amos Heckendorf
The Nest Group, Inc.
508-481-6223
check out this ideas page
http://www.nestgrp.com/idea/idea.shtml
Andy Alpert
PolyLC Inc.
Columbia, MD
tel: (410) 992-5400 FAX: (410) 730-8340 e-mail: PolyLC@aol.com
a nice pesky peptide post (different peptide issues...but a BUNCH of ideas)
http://www.abrf.org/archives/hmail/9820/0051.html
Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software
-----Original Message-----
From: gillespiep <gillespiep@uthscsa.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Tuesday, May 22, 2001 2:15 PM
Subject: peptide purification
I have a peptide that is very hydrophobic that through trial after trial I was
finally successful in synthesizing but now have the tough task of purifying.
It was suggested that I check with the discussion group peptide professionals
and see what suggestions you have on purification. I am checking to see
anyone's experience with a CN or C8 column or using pyridine/propanol on
RP-HPLC. During previous purifications the peptide was lost on the C18
column. If anyone suggests using the above procedures or has one that has
worked better for them then any information is very appreciated. Thanks in
advance.
SEQUENCE: TLVGIIVGVLLAIGF also made with C at N terminus.
Paula Gillespie
Research Assistant
University of Texas Health Science Center
San Antonio, TX 78240
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