disulphide bond prediction

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Hi ABRFers,
I am trying to use protein digests and LC-MS to determine the disulphide
bond arrangements within a couple of smallish knotty proteins.

Does someone know of a web site that possesses a program that can
predict/calculate/indicate the probable disulphide bonds of a (obviously
non-reduced) protein given the mass of peptides from a digest, the
protein's primary sequence, the endoproteinase specificity (and the buffer
conditions of the digest if relevant)?

I am aware of sites and programs that predict the mass of fragments and
products of partial digests, but I need something smarter.

Thanks heaps in advance,
Gene
Gene Wijffels
CSIRO Livestock Industries

Phone: (07) 3346 2510/14
FAX: (07) 3346 2509

Please note new postal address:

CSIRO Livestock Industries,
Level 3 Gehrmann Laboratories,
Research Road
University of Queensland
St Lucia Qld 4072
                

street address: Level 7
                Gehrmann Laboratories
                Research Road
                University of Queensland 4072

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