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This archive was generated by hypermail 2b29
: Wed Nov 21 2001 - 14:16:55 EST
Julie:<br>
<br>
I do not know of a study of the degree of satisfaction of oligo <br>
outsourcing. I suspect that it may be easy to survey but hard to
interpret <br>
the responses, and harder to generalize. These days, with all the vendor
<br>
promotions, those who do not buy from outside may be those who are <br>
consistently pleased with the CORE DNA. If one test outsourcing with only
<br>
a few oligos, the odds favor outsourcing because oligo synthesis succeeds
<br>
over 95% of the time, especially for PCR primers and sequencing primers.
<br>
You will need a large data set.<br>
<br>
At the end of 2000, the Nucleic Acid Research Group of ABRF conducted a
<br>
thorough analysis of the quality of DNA synthesis by about 33 DNA
synthesis <br>
instruments in CORE facilities and commercial facilities . We invite you
<br>
to view the results with a Web browser at <br>
<br>
<font color="#0000FF"><u><a href="ftp://ftp.fccc.edu/yeung/outgoing/" eudora="autourl">ftp://ftp.fccc.edu/yeung/outgoing/><br>
<br>
</font></u>Above NARG study showed that most DNA synthesizers, CORE or
commercial, <br>
make good primers. However, quality varies with respect to whether all
<br>
four nucleotides in DNA are made equally well. Thus the quality of <br>
long oligos and modified oligos may vary greatly among facilities while
<br>
shorter primers may pass. <br>
<br>
Please let the NARG members know if there is something we can do for
you.<br>
If you are making DNA as a service, we like to assure you that with good
quality<br>
control, your DNA will be the best your users can get anywhere.<br>
<br>
Thanks!<br>
<br>
Tony <br>
Chair, NARG.<br>
<br>
<br>
<div>************************************</div>
<div>Anthony T. Yeung, Ph.D.</div>
<div>Director, Fannie E. Rippel Biotechnology Facility</div>
<div>Member, Institute for Cancer Research</div>
<div>Fox Chase Cancer Center</div>
<div>7701 Burholme Ave., Philadelphia, PA 19111</div>
<div>Voice: 215-728-2488 FAX: 215-728-3647 email:
AT_Yeung@FCCC.edu</div>
<div>Public Key: D994 4658 6AE2 12A6 A538 D18C 461F 5ED2 21C5
2AFF</div>
<div><a href="http://www.fccc.edu/research/labs/yeung/" EUDORA=AUTOURL>http://www.fccc.edu/research/labs/yeung/></div>
************************************
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