Hi,
You could try different dilutions of the sample in your matrix solution, until
you get good cristallization.
greetings
Veronique De Brabandere
Dept Protein Analysis
Innogenetics
Belgium
Derek Bradley <Derek.Bradley@ucl.ac.uk> on 29-05-2001 17:58:01
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc: (bcc: Veronique De Brabandere/INNOGENETICS)
Subject: MS: MALDI & DMF
Hi All,
I have a colleague who is asking me to analyse via MALDI, a substance which
is dissolved in DMF and which he swears blind is insoluble in anything
normal for MALDI like acetonitrile or methanol. With the boiling point of
DMF being about 154C I figure it's going to be forever and a day before
even 1ul evaporates normally from the spot so I was hoping someone out
there has had experience of DMF based samples and can offer some useful tips.
I've asked him to try blending the sample with amounts of acetonitrile to
see if this will speed things up without dropping his substance out of
solution. I'm not convinced sticking the plate in an oven for a few hours
is the way to go although using a hairdryer has been mooted....
Any helpful hints will be gratefully received!!
Thanks,
Derek Bradley
Dept. of Medicine
UCL
London
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