Hello
Could someone help me :
1/ Why a Taqman probe is always 5' FAM, HEX or TET and 3' TAMRA (or any
quencher) and not the opposite ? (3' FAM, HEX or TET and 5' TAMRA )
2/ How the 5'>3' exonuclease activity of the polymerase is possible
since the 5'OH end of the probe is blocked because of 5' (FAM, HEX or
TET)
Thank You very much for any answers
BR
Alain
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Alain LAURENT Ph. D
Lab. Manager
DNA&Peptide Chemistry
ESGS Groupe CYBERGENE
Allee christophe Colomb
91 035 EVRY
FRANCE
Tel: +33 (01 60 87 82 00
Fax: +33 (0)1 60 87 82 01
e-mail: a.laurent@eurosequence.com
http://www.eurosequence.com
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