Dear Julie,
Are you using ABI Lane Guide? We experienced something similar and noticed
that the location of the G spikes corresponded to the lane guide bands on
the gel. ABI tech support never came up with a good explanation for why we
were detecting bands which are supposed to be undetectable.
Susan Newman
>Dear Sequencing Core Facilities-
>Does anyone else have trouble with G blobs? We used to have T blobs, so
>we switched from isoprop ppt to Edge columns for post-rxn clean-up before
>running on a 377 gel. That eliminated the problem (mostly) but now we're
>beset by these outrageous G blobs which appear around 50 bp and again
>between 120 bp and 130 bp. The interference gets worse the longer the
>cleaned, dried samples sit before being run (>36hrs). ABI has made
>suggestions about protecting samples from light and controlling humidity,
>but I'd appreciate any feedback or comments from anyone else experiencing
>this phenomena.
>
>PS: We've seen this w/ BigDye ver2 and ver3.
>-Juliane K. Mills
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>Staff Scientist - Sequencing Facility
>DuPont Pharmaceuticals Co.
>E336/243E
>office 302.695.2401
>email Juliane.K.Mills@dupontpharma.com
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