I'm getting ready to look at the biodistribution of a synthetic peptide, and it seems to me that incorporation of tritium-labeled valine is my best bet. Using 3,4-T labeled valine, I would generate a peptide from which exchange of tritium would be extremely unlikely. And I can get it hot enough, and certainly much hotter than with 14-C. And it is much more affordable than other isotopes.
Yet in looking in the literature, I don't find many instances in which tritium-labeled peptides are used for this purpose, and find more 14 C or even 35-S labeled peptides.
Am I missing something here? Is there some reason I have not thought about for avoiding use of tritium for making a radio labeled peptide for use in biodistribution studies?
Any thoughts would be greatly appreciated.
Tom
____________________________
Thomas T. Andersen, Ph.D.
Assistant Dean
Albany Medical College
Albany, NY 12208
518 262-5253
518 262-5183 fax
anderst@mail.amc.edu
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