On Fri, 1 Jun 2001, Dick Winant wrote:
> Hi all,
>
> I wonder if someone can tell me how, in general, peptides behave on
> hplc when they are derivatized with biotin.
>
> Thanks for your help.
>
>
> Dick Winant
> PAN Facility
> Stanford Univ.
>
>
Dear Dick
Biotinylated peptides usually elute one or two minutes later
than their non-biotinylated versions with standard RP solvents/gradients.
Generally they behave as normal peptides but can be less soluble.
Dr Graham Bloomberg
Peptide Synthesis Core Facility
Dept. Biochemistry
Medical School
University of Bristol
Bristol BS8 1TD
UK
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