Insert screen by PCR of Pichia transformed cells.

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Hi to all,
I would like to know if somebody tried the insert screen by PCR of Pichia
transformed cells.
It works on E. coli cells after a denaturating time of 5 minutes at 95
degrees which is necessary to brake the cells; I know it is hard to brake
yeast cells and I think that time at that temperature is not sufficient to
obtain an "exract" of them. I am wondering if I can use in the PCR tubes
some braking buffer or something else. Can I have some advises about it?
Thanks in advance
Maria Lorenzi

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