Re: Digest - staining or not?

From: Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Date: Fri Jun 08 2001 - 14:35:55 EDT


If you can get away without staining, you will get recoveries of some
peptides that are nearly identical to what you get with a solution digest
of the same protein. I've tested this several times. Its the best way
for recovering phosphopeptides--just wait for the radioactivity to decay
before doing the MS.

Katheryn Resing



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