<x-flowed>Just curious--How long do you wait before MS? That is, what level of
radioactivity (32P in particular) is considered safe for MS?
Kara Pearson
NIH/NHLBI
Bethesda, MD 20892
>From: Katheryn Resing <Katheryn.Resing@Colorado.EDU>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Re: Digest - staining or not?
>Date: Fri, 8 Jun 2001 12:35:55 -0600
>
>If you can get away without staining, you will get recoveries of some
>peptides that are nearly identical to what you get with a solution digest
>of the same protein. I've tested this several times. Its the best way
>for recovering phosphopeptides--just wait for the radioactivity to decay
>before doing the MS.
>
>Katheryn Resing
>
>
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</x-flowed>
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