Just my opinion,with no MS experience.The half like of 32P is about 2
weeks,and there is not going to be so much radioactivity in your sample.to
start.You will have little or no radioactivity left,by the time you get to
do MS. The change in mass should be minimal.
Fulvio Perini
UNMC
986805 Nebraska Med.Cntr.
Omaha,NE 68198-6805
"Kara Pearson"
<karapearson@hotm To: Recipients of ABRF List
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06/08/2001 04:10
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Just curious--How long do you wait before MS? That is, what level of
radioactivity (32P in particular) is considered safe for MS?
Kara Pearson
NIH/NHLBI
Bethesda, MD 20892
>From: Katheryn Resing <Katheryn.Resing@Colorado.EDU>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Re: Digest - staining or not?
>Date: Fri, 8 Jun 2001 12:35:55 -0600
>
>If you can get away without staining, you will get recoveries of some
>peptides that are nearly identical to what you get with a solution digest
>of the same protein. I've tested this several times. Its the best way
>for recovering phosphopeptides--just wait for the radioactivity to decay
>before doing the MS.
>
>Katheryn Resing
>
>
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