Recipients of ABRF List <abrf@aecom.yu.edu>
In-Reply-To: <3B2528D5.970F9A93@mail.upstate.edu>
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sender: Association of Biomolecular Resource Facilities <abrf-request@aecom.yu.edu>
Precedence: bulk
Errors-To: abrf-request@aecom.yu.edu
Michael,
You can always try doing your assembly using the
purchased phosphorylated amino acids.
If you have the opportunity, do a kaiser (ninhydrin)
test to ensure your couplings have gone to completion.
If you've already assembled the peptide with
unprotected S and/or T, then you can always try global
phosphorylation techniques. Then, do a test cleave
with a very tiny portion of your peptidyl resin, and
do a MS to confirm/deny completeness. Remember, if
you...for some reason...have unprotected Tyrosine in
your sequence, it'll get phospho'd too.
Regards,
Steven Johnson, B.S. Chem.
Research Associate, Chemistry/Development
Biomeasure, Inc. Milford, MA, USA
--- Michael Curtis <curtism@mail.upstate.edu> wrote:
> Hello.
>
> I am currently working on a phosphorylation project
> and have a question
> concerning neighboring phosphorylation sites. Does
> anyone know of an example of
> two phosphorylateable residues being directly next
> to each other and both being
> phosphorylated? Or sites that are very close?
>
> For example:
>
> Directly next to each other:
>
> i.e XXSTXX giving XXSpTpXX upon phosphorylation
>
> Or very close by:
>
> i.e. XXS(X1-2)TXX giving XXSp(X1-2)TpXX upon
> phosphorylation
>
> I am wondering if phosphorylation of one would
> sterically hinder the
> phosphorylation of the other. Any instance of these
> cases that you know about or
> any insight you have on this issue would be greatly
> appreciated.
>
> Thanks in advance.
>
> Mike
>
> Mike Curtis
> UMU
> curtism@mail.upstate.edu
>
>
__________________________________________________
Do You Yahoo!?
Get personalized email addresses from Yahoo! Mail - only $35
a year! http://personal.mail.yahoo.com/
This archive was generated by hypermail 2b29 : Wed Nov 21 2001 - 14:17:19 EST